Faststart high fidelity pcr system roche manual

Application. Use FastStart Taq DNA Polymerase, GMP Grade, 5 U/μl, for: Hot start PCR and RT-PCR with high specificity, sensitivity and yield. Specific amplification of DNA fragments from various sources of DNA and for diverse down-stream applications. Figure 1: PCR amplification of long templates from nucleic acid samples obtained with the High Pure PCR Template Preparation Kit. Nucleic acids were prepared from blood or cultured human K cells. ng of each preparation was amplified using a tissue-plasminogen-activator (tPA)-specific primer and either the Expand Long Template PCR System. FastStart ™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system.

FastStart™ Taq DNA Polymerase is a thermostable, chemically modified form of recombinant Taq DNA Polymerase. The enzyme is inactive at +15 to +25°C during PCR setup, and then activated at +95°C during initial denaturation. This enzyme delivers superior results due to its unique enzyme design and optimized buffer system. The FastStart High Fidelity PCR System is a blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein, which is also chemically modified. This protein mediates proofreading activity, but carries no polymerase activity. Both proteins are inactive below +75°C and are activated by heating to +95°C for two minutes. FNR_PCR_RZ Uhr Seite 1 Probedruck C M Y CM MY CY CMY K www.doorway.ru PCR Applications Manual 3rd edition.

Dec Extraction protocol, For epithelial cell preparation from colon, using the Fast Start High Fidelity PCR System (Roche) with buffer 2. Dec Primers ESFor and ESRevB were used to amplify the DNA from both strains using the Expand high fidelity PCR system (Roche Diagnostics). Jan We also tested a modified PCR protocol, which has been suggested to reduce Six enzymes (Phusion, Gold, FastStart, Roche Taq, HotStar and.